In vitro antioxidant activity thesis

The absorbance reading was taken after 6 minutes following the reaction. Total antioxidant potential and polyphenol content of the herbal preparation was measured.

High amounts of free radical molecules cause oxidative stress in cells which result in damaging essential macromolecules including DNA, lipids, and proteins.

Red wines were analysed for their phenolic content and antioxidant activity using a range of complementary techniques including HPLC-tandem mass spectrometry, preparative HPLC and HPLC with an on-line antioxidant detection system.

A clear understanding of the antioxidant properties of this herbal preparation will contribute towards evaluating its efficacy and make appropriate recommendation for its use. Literature search revealed that essential oils from R. The leaf, root, and seed oil of the plant have also been used for treatment of inflammation and liver disorders in India [ 15 ].

HPLC analysis showed the presence of quercetin, apigenin, kaempferol and luteolin in the supplement.

Medicinal plants are rich in active phytochemical compounds with various biological activities. Flavonoids are a group of polyphenolic compounds found abundantly in the plant kingdom.

Hence, cytotoxic level of medicinal plants must also be evaluated against host cells. Secondary metabolites are responsible for biological activities of plants including terpenoids [ 11 ], phenolic compounds flavonoids, phenolic acids, quinones, coumarins, lignans, stilbenes, tanninsand nitrogen compounds alkaloids, amines, and betalains and carotenoids [ 12 ].

The safety of plants as a potential therapeutically agents must be ascertained and the side effects should be acceptable to the host. Blood samples were analyzed at baseline and at the conclusion of the treatment period for total antioxidant capacity, polyphenol content, protein, lipid and LDL oxidation, and the level of glutathione peroxidase.

The use of medicinal plants has increased in rural areas and developing countries. Reactive oxygen species are also reported as carcinogenic and mutagenic agents [ 9 ]. Chromatograms were examined under ultraviolet light and nm and sprayed with freshly prepared vanillin spray reagent 0.

Medicinal plants continue to provide humanity with new remedies. Plant was confirmed by Dr. HPLC-MS2 revealed the presence of a number of flavoids and phenolic compounds of which 19 were identified, with gallic acid, the flavinols and anthocyanins being the most abundant.

These drugs are known to cause severe side effects in the body such as heart attacks and stroke [ 10 ]. HPLC with an on-line antioxidant detection system was subsequently used to separate and identify red wine phenolics. The flavonols and anthocyanins were minor antioxidant components in red wines.

Voucher specimens in the garden Herbarium and tree labels verified the identity of the plant. It is therefore important to explore medicinal plants for their safety, quality, toxicity, appropriate amount of plant materials to use, and efficacy.

Interest in the possible health benefits of flavonoids and other polyphenolic compounds has increased in recent years owing to their potent antioxidant and free-radical scavenging activities [ 6 — 12 ].

In vitro studies were conducted using a liposome model system to simulate biological cell membranes.

This article has been cited by other articles in PMC. Yellow zones appearing on the chromatograms indicate the presence of scavenging activity of free radicals by compounds presence in plant extracts [ 22 ].

Antioxidants play an important role in neutralizing free radical species which are produced as end or by-products of normal biochemical reactions in normal system [ 6 ].

Published online Aug 3. By combining HPLC, MS2 and on-line assessment of antioxidant activity, the major phenolic compounds present in red wine were identified, together with their direct contribution to the total antioxidant activity. The process was repeated three times to exhaustively extract the plant material and the extracts for each solvent were combined.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The leaves of R. All determinations were carried out in triplicate and calculated as follows: The plant was selected based on the high antioxidant activity demonstrated in previous in vitro study [ 20 ].

Abstract Ricinus communis has been utilized traditionally as medicine to treat inflammatory related diseases including wounds, sores, and boils. Preparative HPLC was used in an effort to isolate the antioxidant components in red wine and 60 aliquots were collected.

Plant constituents are responsible for both free radicals scavenging and anti-inflammatory activity.zoriŢa maria sconŢa extraction, purification, characterization and in vitro testing of anthocyanin rich fractions obtained from aronia melanocarpa and vaccinium sp.

phd thesis abstract phd supervisor prof. carmen socaciu, phd. 88 Research Article THE IN VITRO ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF FOUR INDIAN MEDICINAL PLANTS MARYAM ZAHIN1, FARRUKH AQIL2 AND IQBAL AHMAD*1 1Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

2Brown Cancer Center, B Delia Baxter. An-Najah National University Faculty of Graduate Studies In-vitro evaluation of acetylcholinesterase inhibition and antioxidant activity of selected Palestinian medicinal plants: Implications.

PhD Thesis Antioxidant properties of extracts from selected plant materials (Caesalpinia spinosa, Perilla frutescens, Artemisia annua and Viola wittrockiana) in vitro and in model food systems Monika Skowyra Supervisor: Dr.

Antioxidant activity, protective effects and absorption of polyphenolic compounds

María Pilar Almajano Pablos. Antioxidant activity of oat bran hydrolyzed proteins in vitro and in vivo by Sara Jodayree A thesis submitted to the Faculty of Graduate and Postdoctoral. In Vitro Assessment of Cytotoxicity, Antioxidant, and Anti-Inflammatory Activities of Ricinus communis (Euphorbiaceae) Leaf Extracts.

Vhutshilo Nemudzivhadi and Peter Masoko * dichloromethane, acetone, and methanol using serial exhaustive extraction method. Antioxidant activity of all crude extracts was quantitatively measured against .

In vitro antioxidant activity thesis
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